Anti-inflammatory effect of Phytomarine R-L compound against lipopolysaccharide- induced pulmonary injury in BALB/c mice

Background: This study deals with the induction of lipopolysaccharide induced inflammation in animal model and treating this condition using phytomarine R-L compound (Bloomin’Age, Science Living, Milan Italy) that is known to possess anti-inflammatory properties eventually determine their characteristics through in-vivo experimentation.Objective study:The present was subjected test therapeutic anti-inflammatory/anti-oxidative regenerative property (SBF-LF) an LPS-induced lung injury mice models. Methods:Mice received intra-nasal administration LPS at a concentration 1 mg/ml weekly per day week for four consecutive weeks followed by oral 50 mg/kg body weight intermittently. Broncho-alveolar lavage fluids (BALF) as well tissues were periodically collected from control experimental groups histological examination. Likewise, tissue homogenate obtained treatment assayed myeloperoxidase (MPO) assay, superoxide dismutase (SOD) activity elastase assay. Results: The results illustrated LPS- group demonstrated increased inflammatory cell infiltration causing acute persisting neutrophilic accumulation parenchyma alveolar congestion. Furthermore, influx macrophages, lymphocytes mediators observed during BALF analysis. resulted significant decline permeation cells histology Conclusion: Thus, exploitation along other assay methods confirmed its robust antioxidant suggested epithelial capacity. Further analyzing efficacy might help understanding potential option clinical settings.Keywords:Lungs, BALB/c mice, Lipopolysaccharide, anti-inflammatory,